Journal:

Article Title: Elevated Neuronal Expression of CD200 Protects Wld s Mice from Inflammation-Mediated Neurodegeneration

doi: 10.2353/ajpath.2007.060677

Figure Lengend Snippet: CD200R expression in Wlds and WT splenocytes and CNS. CD200R expression was assessed by flow cytometry in WT and Wlds splenocyte populations on day 0 (a) and at day 15 (b) after immunization. CD200R was predominantly found on naïve CD11b+ and CD11c+ cells in both strains with no significant difference between the WT and Wlds strains. Expression of CD200R decreased after immunization on these cells. c: Protein levels were assessed in spinal cord lysates from WT and Wlds mice on day 0 and day 15 after immunization, by Western blot, and expressed as protein/β-actin IDV values. There was no significant difference in CD200R expression between the strains. IL-6 was reduced and IL-10 was elevated in Wlds mice compared with WT mice after immunization at day 15 after immunization. There was no significant difference in tumor necrosis factor-α, TLR-4, or CD22 expression between the two strains at either time point. d: Fractalkine (CX3CL1) concentration in spinal cord homogenates from WT and Wlds at day 0 and day 14 after immunization was assessed using ELISA assay. Results from four mice/group/time point were averaged. There was no significant difference between WT and Wlds mice at either time point.

Article Snippet: Antibodies Used for Immunofluorescence Staining The following antibodies were used: CD200 (clone 3B6, isotype rat IgM, 1:200; Cedarlane Laboratories, Hornby, ON, Canada); secondary: Alexa 488-conjugated goat anti-rat IgM (Molecular Probes); CD200R (anti-313015 CD200R peptide, 26 clone R252, isotype rabbit IgG, 1:200; Trillium Therapeutics Inc.); secondary: Alexa 594-conjugated goat anti-rabbit IgG (Molecular Probes); CD200R (clone OX-110, isotype rat IgG2a, 1:100; Serotec, Oxford, UK); secondary: Alexa 488-conjugated rabbit anti-rat IgG (Molecular Probes); mitogen-activated protein 2 (MAP-2) (clone HM-2: mouse anti-mouse IgG1, 1:100; Sigma); secondary: Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); NeuN (clone A60, isotype mouse IgG1, 1:100; Chemicon/Millipore, Temecula, CA); secondary: Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); GFAP cocktail (clones 4A11, 1B4, 2E1, isotype mouse IgG2b, 1:100; BD Pharmingen, Palo Alto, CA); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); CNPase (clone 11-5B, isotype mouse IgG1, 1:100; Chemicon/Millipore); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); β-tubulin (clone TUJ1, isotype mouse IgG2a; 1:100; Covance, Berkeley, CA); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); CD4 (clone H29.129; isotype rat IgG2a; 1:50; BD Pharmingen); secondary: Alexa-488-conjugated goat anti-rat IgG (Molecular Probes); and CD8 (clone 53-6.7; isotype rat IgG2a, 1:50; BD Pharmingen); secondary: Alexa-488-conjugated goat anti-rat IgG (Molecular Probes).

Techniques: Expressing, Flow Cytometry, Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Elevated Neuronal Expression of CD200 Protects Wld s Mice from Inflammation-Mediated Neurodegeneration

doi: 10.2353/ajpath.2007.060677

Figure Lengend Snippet: CD200R is expressed on microglia, oligodendrocytes, and astrocytes. Spinal cord sections from WT and Wlds mice on days 0, 22, and 60 after immunization were double-stained with CD200R (red) and LB4-microglial or GFAP (astrocytes) or CNPase (oligodendrocytes) or β-tubulin (axons and neurons). Shown are representative merged confocal images from mice on day 22 after immunization. CD200R staining was present on microglia/macrophages as well as astrocytes and oligodendrocytes from both strains. CD200R was not present on axons/neurons. Original magnifications, ×63.

Article Snippet: Antibodies Used for Immunofluorescence Staining The following antibodies were used: CD200 (clone 3B6, isotype rat IgM, 1:200; Cedarlane Laboratories, Hornby, ON, Canada); secondary: Alexa 488-conjugated goat anti-rat IgM (Molecular Probes); CD200R (anti-313015 CD200R peptide, 26 clone R252, isotype rabbit IgG, 1:200; Trillium Therapeutics Inc.); secondary: Alexa 594-conjugated goat anti-rabbit IgG (Molecular Probes); CD200R (clone OX-110, isotype rat IgG2a, 1:100; Serotec, Oxford, UK); secondary: Alexa 488-conjugated rabbit anti-rat IgG (Molecular Probes); mitogen-activated protein 2 (MAP-2) (clone HM-2: mouse anti-mouse IgG1, 1:100; Sigma); secondary: Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); NeuN (clone A60, isotype mouse IgG1, 1:100; Chemicon/Millipore, Temecula, CA); secondary: Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); GFAP cocktail (clones 4A11, 1B4, 2E1, isotype mouse IgG2b, 1:100; BD Pharmingen, Palo Alto, CA); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); CNPase (clone 11-5B, isotype mouse IgG1, 1:100; Chemicon/Millipore); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); β-tubulin (clone TUJ1, isotype mouse IgG2a; 1:100; Covance, Berkeley, CA); secondary: Alexa 488- or Alexa 594-conjugated goat anti-mouse IgG (Molecular Probes); CD4 (clone H29.129; isotype rat IgG2a; 1:50; BD Pharmingen); secondary: Alexa-488-conjugated goat anti-rat IgG (Molecular Probes); and CD8 (clone 53-6.7; isotype rat IgG2a, 1:50; BD Pharmingen); secondary: Alexa-488-conjugated goat anti-rat IgG (Molecular Probes).

Techniques: Staining

Journal: Oncology Letters

Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only

doi: 10.3892/ol.2017.6592

Figure Lengend Snippet: Primary antibodies used in the study.

Article Snippet: Glycodelin , Mouse (IgG1κ) , 6F2 , 116–0646 , Zytomed Systems GmbH, Berlin, Germany , 1:4,000 in PBS.

Techniques:

Journal: Oncology Letters

Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only

doi: 10.3892/ol.2017.6592

Figure Lengend Snippet: Expression of the different proteins in the different groups.

Article Snippet: Glycodelin , Mouse (IgG1κ) , 6F2 , 116–0646 , Zytomed Systems GmbH, Berlin, Germany , 1:4,000 in PBS.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only

doi: 10.3892/ol.2017.6592

Figure Lengend Snippet: Glycodelin expression in Group A (patients with endometrioid endometrial cancer and adenomyosis uteri). Representative microphotographs of glycodelin immunohistochemical staining are shown (immunoreactivity score, 4).

Article Snippet: Glycodelin , Mouse (IgG1κ) , 6F2 , 116–0646 , Zytomed Systems GmbH, Berlin, Germany , 1:4,000 in PBS.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Oncology Letters

Article Title: Coexistence of adenomyosis uteri and endometrial cancer is associated with an improved prognosis compared with endometrial cancer only

doi: 10.3892/ol.2017.6592

Figure Lengend Snippet: Glycodelin expression in Group B (patients with endometrioid endometrial cancer only). Representative microphotographs of glycodelin immunohistochemical staining are shown (immunoreactivity score, 6).

Article Snippet: Glycodelin , Mouse (IgG1κ) , 6F2 , 116–0646 , Zytomed Systems GmbH, Berlin, Germany , 1:4,000 in PBS.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Reproductive Medicine and Biology

Article Title: Upregulated serum granulysin levels in women with antiphospholipid antibody‐associated recurrent miscarriage are downregulated by heparin treatment

doi: 10.1002/rmb2.12460

Figure Lengend Snippet: Serum granulysin concentration in women with or without antiphospholipid antibodies

Article Snippet: Briefly, microtiter plates (Nunc) were coated with 5 mg/ml anti‐granulysin monoclonal (m)Ab RB1(Mouse IgG1κ) (MBL International Corporation) in 100 mM carbonate buffer and maintained overnight at 4°C.

Techniques: Concentration Assay

Journal: Reproductive Medicine and Biology

Article Title: Upregulated serum granulysin levels in women with antiphospholipid antibody‐associated recurrent miscarriage are downregulated by heparin treatment

doi: 10.1002/rmb2.12460

Figure Lengend Snippet: Changes in serum granulysin levels in patients with RPL treated with heparin and aspirin. Serum granulysin concentration was assayed using ELISA. (a) Serum granulysin levels of patients treated with heparin plus aspirin combination therapy were measured before and 1 week after the initiation of heparin treatment ( n = 32). (b) Serum granulysin levels of patients treated with aspirin alone ( n = 30). The gestational age of sampling is the same as in the heparin plus aspirin combination therapy group. *** p < 0.001: paired t test

Article Snippet: Briefly, microtiter plates (Nunc) were coated with 5 mg/ml anti‐granulysin monoclonal (m)Ab RB1(Mouse IgG1κ) (MBL International Corporation) in 100 mM carbonate buffer and maintained overnight at 4°C.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Sampling

Journal: Reproductive Medicine and Biology

Article Title: Upregulated serum granulysin levels in women with antiphospholipid antibody‐associated recurrent miscarriage are downregulated by heparin treatment

doi: 10.1002/rmb2.12460

Figure Lengend Snippet: Changes in serum granulysin levels in patients with PE‐positive and PE‐negative RPL. Serum granulysin concentration was assayed using ELISA. (a, b) Serum granulysin levels of PE‐positive (a) and PE‐negative (b) patients treated with heparin plus aspirin were measured before and 1 week after the initiation of heparin treatment ( n = 19 and 13, respectively). (The gestational age of sampling is the same as in the heparin plus aspirin combination therapy group.) ** p < 0.01: paired t test

Article Snippet: Briefly, microtiter plates (Nunc) were coated with 5 mg/ml anti‐granulysin monoclonal (m)Ab RB1(Mouse IgG1κ) (MBL International Corporation) in 100 mM carbonate buffer and maintained overnight at 4°C.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Sampling

Journal: Reproductive Medicine and Biology

Article Title: Upregulated serum granulysin levels in women with antiphospholipid antibody‐associated recurrent miscarriage are downregulated by heparin treatment

doi: 10.1002/rmb2.12460

Figure Lengend Snippet: Pregnancy outcome stratified by granulysin levels after heparin plus aspirin treatment. ** p < 0.05: Fisher's exact probability test

Article Snippet: Briefly, microtiter plates (Nunc) were coated with 5 mg/ml anti‐granulysin monoclonal (m)Ab RB1(Mouse IgG1κ) (MBL International Corporation) in 100 mM carbonate buffer and maintained overnight at 4°C.

Techniques: